■ 基本信息
原核抗性: | Amp |
真核抗性: | HIS3 |
| 克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質粒屬性
質粒宿主: | 酵母菌 |
質粒用途: | 雜交 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | HIS3
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熒光標記: |
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■ 質粒簡介
pGilda is a cloning vector used to generate fusions of a bait protein with the 202-residue LexA protein, which acts as a DNA-binding domain in the MATCHMAKER LexA Two-Hybrid System . The hybrid protein is expressed in yeast cells from the tightly regulated GAL1 promoter (PGAL1); transcription is repressed in the presence of glucose and induced in the the absence of glucose and presence of galactose . pGilda carries the HIS3 gene for selection in His– auxotrophic yeast strains and is compatible with MATCHMAKER LexA Libraries. This vector replicates autonomously in both E. coli (using the Col E1 ori) and in S. cerevisiae (using the CEN/ARS ori). Ampr is used for selection in E. coli.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1778 pGilda酵母雙雜交質粒.txt
