■ 基本信息
別名: | pGADT7-AD |
啟動子: | ADH1 |
復制子: | 2μ |
質粒分類: | 酵母系列，酵母雙雜交載體 |
質粒大小: | 7987bp |
原核抗性: | Amp |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 酵母細胞 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物:
| 根據(jù)序列設計引物
|
備注: | 酵母雙雜交空載體 |
■ 質粒屬性
質粒宿主: | 酵母菌 |
質粒用途: | 雜交 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | LEU2
|
熒光標記: |
|
■ 質粒簡介
pGADT7 AD is a yeast expression vector that is designed to express a protein of interestfused to a GAL4 activation domain (AD; amino acids 768–881). Transcription of the GAL4 ADfusion is driven by the constitutively active ADH1 promoter (PADH1), and is terminated at theADH1 transcription termination signal (TADH1). The GAL4 AD fusion contains an N-terminalSV40 nuclear localization signal (SV40 NLS; 1) that targets the protein to the yeast nucleus,and a hemagglutinin epitope tag (HA Tag), located between the GAL4 AD and the proteinof interest, that allows the protein to be easily detected with HA-tag antibodies.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK974pGADT7（別名pGADT7-AD）酵母雙雜交質粒.txt
