■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal,BGH poly(A) signal |
質(zhì)粒大小: | 5159bp |
原核抗性: | Amp |
真核抗性: | Zeo |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物: | BGH:TAGAAGGCACAGTCGAGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá),分泌表達(dá) |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: | Zeo
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pSecTag2 A, B, and C vectors are fusion vectors requiring that you clone your gene of interest in frame with the initiation ATG of the N-terminal Igκ-chain leader sequence and/or the C-terminal myc epitope/polyhistidine tag. Three versions of this vector are provided to facilitate cloning. For proper expression,first determine which restriction sites are appropriate for ligation and then which vector will preserve the reading frame at BOTH the 5′ and the 3′ ends. It may be nece ssary to PCR your gene product to create a fragment with the appropriate restriction sites to clone in frame at both ends. Carefully inspect your gene and the multiple cloning site of each vector before cloning your gene of interest.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK690pSecTag2A哺乳分泌質(zhì)粒.txt
